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Fig. 1 | Molecular Pain

Fig. 1

From: The opioid peptide dynorphin A induces leukocyte responses via integrin Mac-1 (αMβ2, CD11b/CD18)

Fig. 1

Screening of the peptide library spanning the sequence of dynorphin AB for αMI-domain binding. a, Amino acid sequence of dynorphin AB (residues 1–32). b, Cellulose membrane with the assembled peptide library consisting of 9-mer peptides with three-residue offset was incubated with 125I-labeled αMI-domain and subjected to autoradiography followed by densitometry. The αMI-domain binding was observed as dark spots. Spot #10 contains only the β-Ala spacer. The αMI-domain binding was expressed as a percentage of binding to peptide #1, which was assigned a value 100 % and is shown as the numbers on the right of each sequence. The peptide energies that serve as a measure of probability each peptide can interact with the αMI-domain were calculated as described [17]

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Molecular Pain

ISSN: 1744-8069

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